Left: Visualization of tyrosine hydroxylase expression in rat brain by Immunofluorescent analysis. Section was stained with rabbit anti-TH antibody, R-2120-100, (red, 1:10,000); costained with mouse antibody to pNF-H (green, 1:1,000); and Hoechst staining of nuclear DNA (blue). Following transcardial perfusion of rat with 4% paraformaldehyde, the brain was post fixed for 24 hours and cut into 45µM free floating sections for staining. Product R-2120-100 stains the striatal TH expressing interneurons. Right: Western blot analysis of tissues and lysates using R-2120-100 (green, 1:5,000) with a band at approx. 60kDa in brain and PC12 extracts. Lane 1: protein standard (red); Lane 2: rat brain; Lane 3: mouse brain; Lane 4: SH-SY5Y cells; Lane 5: PC12 cells.