Product DescriptiongoogleRabbit anti-Neurturin (NTN) Polyclonal Antibody (Unconjugated), suitable for WB.
Application(s)WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityAntibody does not show cross-reactivity to other GDNF-family proteins.
Species ReactivityHuman
Immunogen DescriptionRecombinant human NRTN protein produced using CHO-based suspension cell line. Protein was purified from the cell culture supernatant.
Product DescriptionRabbit anti-Neurturin (NTN) Polyclonal Antibody (Unconjugated), suitable for WB.
Application(s)WB
Application DetailsWestern blot (WB) at a suggested dilution of 1:500-1:1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetNeurturin (NTN)
SpecificityAntibody does not show cross-reactivity to other GDNF-family proteins.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionRecombinant human NRTN protein produced using CHO-based suspension cell line. Protein was purified from the cell culture supernatant.
Purity DescriptionProtein G purified IgG
FormatConcentrated ammonium sulphate in PBS pH 7.4.
Storage InstructionsStore at 2-8°C upon receipt. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundNeurturin (NTN) is a member of the GDNF family of neurotrophic factors. This protein is a potent survival factor for several populations of central and peripheral neurons in mature and developing rodents. FUNCTION: Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. DISEASE: Defects in NRTN are a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, and possibly with other loci, defects in NRTN are involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Western-Blot detection of human NRTN expressed in CHO cells. Line 1. hNRTN-containing CHOEBNALT85 cell culture supernatant. Line 2. Negative control - CHOEBNALT85 cell culture supernatant. 5 µl of supernatant were loaded per line. Primary antibody dilution 1:5000 was used. Antibody recognizes protein dimeric, and monomeric variants.