Rat cerebellum section stained with chicken anti-parvalbumin (green, 1:2,000) and mouse anti-GFAP (red) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free floating sections were stained. The parvalbumin chicken antibody labels the perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia and astrocytes.