Image shows rat mixed neuron/glial cultures stained with Chicken polyclonal antibody to Peripherin C-1399-50 (green channel) and Rabbit polyclonal antibody to Internexin alpha R-1379-50 (green channel). These cultures contain mostly neurons which are rich in internexin-alpha, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of peripherin and internexin-alpha, the green and red signals superimpose to produce a golden cell. Blue is a DNA stain.
Left: Analysis of peripherin expression in mixed fibroblast and PC12 pheochromocytoma cell culture by Immunocytochemistry. Cells were stained with chicken antibody to peripherin (1:5,000, green), and co-stained with rabbit antibody to vimentin (R-1699-100, 1:2,000, red). Blue: DAPI nuclear stain. The peripherin antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while the vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin. Right: Western blot analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) with chicken antibody to peripherin (1:10,000). [1] protein standard, [2] rat, [3] mouse, [4] pig, [5] cow, [6] SH-SY5Y, [7] PC12. The band at 57 kDa corresponds to peripherin protein.