Analysis by flow cytometry of Neurofilament heavy polypeptide, phosphorlylated (pNF-H), (endogenous) expression in mouse neural progenitor cells differentiated from mES and fixed overnight in 70% ethanol (Red curve). PE-labelled goat anti-mouse IgG was used as secondary antibody. Negative control processed with secondary antibody only (Blue curve). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
Analysis by western blot of pNF-H expression in tissue lysates with M-1387-50, Neurofilament heavy polypeptide, phosphorylated (pNF-H), Clone NAP4, Mouse mAb, (green, 1:10,000) with a strong band at approx 200-220kDa corresponding to the phosphorylated form of the NF-H subunit. A minor band is observed at 160kDa corresponding to the non-phosphorylated NF-H. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations. Lane 1: MWM; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord; Lane 6: pig spinal cord; Lane 7: cow spinal cord.