Image of a rat hippocampus section by Immunofluorescence and Immunohistochemistry. The section was stained with C-2127-100, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Chicken pAb, (red, 1:5,000), and co-stained with productR-1820-50, Ionized calcium-binding adapter molecule 1 (IBA1), Rabbit pAb, (1:2,000, green). Product C-2127-100 stains the nuclei and distal perikarya of most neurons, while product R-1820-50 labels microglial cells. IHC Method: Following transcardial perfusion with 4% paraformaldehyde, rat brain was post fixed for 24 hours, cut to 35µm, and free floating sections were stained.