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LR3IGF-I, Human, Rapid™ ELISA assay
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US$398.50
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Catalog Number
BEK-2233
- Product Name LR3IGF-I, Human, Rapid™ ELISA assay
-
Product Description
Human LR3 insulin-like Growth Factor-I (LR3IGF-I) was developed by GroPep Bioreagents(www.gropep.com) specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis.
This LR3IGF-I Rapid ELISA kit combines GroPep's many years of expertise in the field of IGF research and Biosensis' newly established Rapid ELISA platform. This collaboration has resulted in the new LR3IGF-I Rapid ELISA kit, which provides for the sensitive, specific and reliable quantification of LR3IGF-I protein in less than 3 hours!
The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR3IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR3IGF-I capture antibody pre-coated onto an ELISA plate, standard LR3IGF-I protein, a biotinylated anti- LR3IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR3IGF-I present in samples and the supplied protein standard.
This LR3IGF-I Rapid ELISA kit has been developed, optimized and validated to quantify LR3IGF-I protein in cell culture medium. It is likely to be used to measure LR3IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. - Alternative Names Human LR3 insulin-like Growth Factor-I.
- Application(s) ELISA
- Specificity Human The assay is intended for quantification of LR_IGF-I. Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
- Species Reactivity Human
- Immunogen Description Recombinant LR3-IGF1 protein, made in E.coli
- Range 3.9 ng/mL - 200 ng/mL
- Sensitivity Typical limit of detection (LOD) for LR_IGF-I is 1 ng/mL determined as 150% of the blank value.
- Regulatory Status For research use only.
Product Info
-
Product Description
Human LR3 insulin-like Growth Factor-I (LR3IGF-I) was developed by GroPep Bioreagents(www.gropep.com) specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis.
This LR3IGF-I Rapid ELISA kit combines GroPep's many years of expertise in the field of IGF research and Biosensis' newly established Rapid ELISA platform. This collaboration has resulted in the new LR3IGF-I Rapid ELISA kit, which provides for the sensitive, specific and reliable quantification of LR3IGF-I protein in less than 3 hours!
The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR3IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR3IGF-I capture antibody pre-coated onto an ELISA plate, standard LR3IGF-I protein, a biotinylated anti- LR3IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR3IGF-I present in samples and the supplied protein standard.
This LR3IGF-I Rapid ELISA kit has been developed, optimized and validated to quantify LR3IGF-I protein in cell culture medium. It is likely to be used to measure LR3IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. - Application(s) ELISA
- Application Details ELISA. For the quantification of LR3IGF-I in Culture Supernatant. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target LR3IGF-I
- Specificity Human The assay is intended for quantification of LR_IGF-I. Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
- Target Cross-Reactivity (ELISA) Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description Recombinant LR3-IGF1 protein, made in E.coli
- Range 3.9 ng/mL - 200 ng/mL
- Sensitivity Typical limit of detection (LOD) for LR_IGF-I is 1 ng/mL determined as 150% of the blank value.
- Sample Type Culture Supernatant
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 12 months from purchase.
- Alternative Names Human LR3 insulin-like Growth Factor-I.
- Uniprot Number P05019 (unmodified)
- Uniprot Number/Name P05019 (IGF1_HUMAN), unmodified
- Scientific Background LR_IGF-I is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
